Onion Lab Report

Introduction:
Discuss three types of water pollution and what are the causes of the pollution.
Explain the purpose of the experiment and put it in the context of what you learned about water pollution.

Materials and Methods: These directions were previously given to you.

Materials:
List all the items you are using to perform this experiment:
For example:
50ml centrifuge tubes

Methods: Write down what you did:
a. How did you make the solutions:

• 0.1% Bleach-1ml of bleach in 1000ml of distilled water
• 0.1% Drano -1 ml Drano in 1000 ml of distilled water
• 0.1% Shampoo-1 ml shampoo in 1000 ml of distilled water
• 0.1% Road Salt-1 gram of salt in 1000 ml water
• 0.1% Aspirin 0.1g of aspirin in 1000ml of distilled water
• 0.1%Fertilizer-0.1g of fertilizer in 1000ml  of distilled water
• Each table will prepare one of the solutions. All the solutions will be tested by each table.

b. How did you prepare the onions?
c. How much solution was placed in each centrifuge tube?

Example: Onions were placed on 50 ml centrifuge tubes containing the control and test solutions so that the base of the onion was suspended in the solution.  You could draw a picture. The tubes were than placed in a rack and put---------where? Light or no light??
Temperature??

Results:
1. State your results. Use a “BEFORE and AFTER” sheet to organize your reported results.

• State the null and alternative hypotheses.
• State your confidence level.
• Show your calculations and/or TI steps for the t-test and t-intervals you choose to do.
• Report the results of the t-test in the language of statistics.
• Report the results of the t-intervals in the language of statistics.
• Include an appropriate graph or graphs of the data.

Conclusion:
a. Interpret the meaning of your results. Did any of the chemicals inhibit root growth, did any of them stimulate root growth?
b. Were the differences between the control and experimentals statistically significant? 
c. Where you surprised by any of your results? Explain
d. Do your road salt results make sense when compared to what is written in the literature about road salt.
e. What is eutrophication? Do any of your results support the ideas of eutrophication?
f. Based on your results what additional experiments could you do to gain more information?(state at least 2 experiments)

References:
Water pollution
http://mbgnet.mobot.org/fresh/pollute.htm

http://www.epa.gov/safewater/privatewells/booklet/humanactivities.html

Road salt
http://www.ec.gc.ca/science/sandejan02/article3_e.html

Wastewater
http://water.usgs.gov/pubs/fs/2004/3127/index.html

Eutrophication
http://toxics.usgs.gov/definitions/eutrophication.html

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